Difference between revisions of "Part:BBa K4583018"

 
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==Usage and Biology==
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===QS system===
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Quorum sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.
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===Esa I/R system===
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The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--<i>Pantoea stewartii</i> subsp. <i>stewartia</i>. EsaR can act as both transcriptional activator and repressor. PesaR is a natural EsaR-repressed promoter, whereas PesaS is a natural EsaR-activated promoter. At low cell density (low ρ), EsaR binds to its esa box to turn off PesaR and turn on PesaS. In the presence of AHL, EsaR can bind to AHL and release from the DNA. Thus, at high cell density(high ρ), the PesaR is turned on and the PesaS is turned off[2].
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<html>
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<figure>
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  <img src="https://static.igem.wiki/teams/4583/wiki/esa.png"width="450" height="290">
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  <figcaption><b>Fig. 1 </b>. schematic illustration of Esa I/R system </figcaption>
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</figure>
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</html>
  
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__NOTOC__
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===PesaRwt===
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<partinfo>BBa_K4583018 short</partinfo>
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PesaRwt refers to the wild-type PesaR.
  
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PesaRwt-RBS-mkate
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4583018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4583018 SequenceAndFeatures</partinfo>

Revision as of 12:03, 5 October 2023

Usage and Biology

QS system

Quorum sensing (QS) is a natural form of cell-cell communication that regulates the metabolic behaviour of bacteria based on changes in their local cell density. As cell density increases, signalling molecules accumulate and are sensed by QS-controlled gene expression regulators, which turn on relevant gene expression.

Esa I/R system

The Esa I/R system is quite special from traditional QS system. The EsaI/R QS system is homologous to the LuxI/R QS system and originates the maize pathogen--Pantoea stewartii subsp. stewartia. EsaR can act as both transcriptional activator and repressor. PesaR is a natural EsaR-repressed promoter, whereas PesaS is a natural EsaR-activated promoter. At low cell density (low ρ), EsaR binds to its esa box to turn off PesaR and turn on PesaS. In the presence of AHL, EsaR can bind to AHL and release from the DNA. Thus, at high cell density(high ρ), the PesaR is turned on and the PesaS is turned off[2].

Fig. 1 . schematic illustration of Esa I/R system

PesaRwt

PesaRwt refers to the wild-type PesaR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 281
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 946
    Illegal SapI.rc site found at 328