Difference between revisions of "Part:BBa K4887003:Design"

 
 
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===Design Notes===
 
===Design Notes===
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It is the FLAG geng harboured in the backbone of plasmid psgR-Cas9-At.The transgenic lines can be detected by western blot using FLAG antibody.
 
+
  
  
 
===Source===
 
===Source===
 +
3*FLAG is synthesized according to Wenshan Liu et al. (2015)
  
......
 
  
 
===References===
 
===References===
 +
Wenshan Liu, Xiaohong Zhu, Mingguang Lei, Qingyou Xia, JR Botella, Jian-Kang Zhu, Yanfei Mao. A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis. Science Bulletin, 2015, 15(60).

Latest revision as of 06:13, 5 October 2023


3*FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is the FLAG geng harboured in the backbone of plasmid psgR-Cas9-At.The transgenic lines can be detected by western blot using FLAG antibody.


Source

3*FLAG is synthesized according to Wenshan Liu et al. (2015)


References

Wenshan Liu, Xiaohong Zhu, Mingguang Lei, Qingyou Xia, JR Botella, Jian-Kang Zhu, Yanfei Mao. A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis. Science Bulletin, 2015, 15(60).