Difference between revisions of "Part:BBa K4907000"

Revision as of 04:57, 5 October 2023

sfibp-his tag

Biology

https://static.igem.wiki/teams/4907/wiki/parts/k4907000.png?w=200&h=200

sfibp

SfIBP is a kind of antifreeze proteins (AFPs) found from Shewanella frigidimarina (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as Thermal Hysteresis (TH) and ice recrystallization inhibition (IRI) respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs.

Usage and Design

In order to measure TH and IRI activity of SfIBP, and finally verify if it is capable to solve soil frost problem, a His-tag (6×his) was added to the C-terminal of SfIBP for purification. We constructed this part and assembled it on the expression vector pET-28a(+).

123

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 634
Illegal AgeI site found at 649
1000
COMPATIBLE WITH RFC[1000]

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 ===Biology===
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 ====sfibp====
 SfIBP is a kind of antifreeze proteins (AFPs) found from <i>Shewanella frigidimarina</i> (1). It is capable to prevent liquid from freezing within a certain temperature range and inhibit ice growth, which is defined as <b>Thermal Hysteresis (TH)</b> and <b>ice recrystallization inhibition (IRI)</b> respectively. Additionally, the IRI activity of SfIBP is much higher beyond other AFPs.