Difference between revisions of "Part:BBa K260009:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K260009 short</partinfo> | <partinfo>BBa_K260009 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
− | The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone '''pMA-RQ-@TetFlp''' to pTetFlp [[Part:BBa_K260002|]] via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched. | + | The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone '''pMA-RQ-@TetFlp''' to pTetFlp [[Part:BBa_K260002|BBa_K260002]] via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched. |
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===Source=== | ===Source=== | ||
− | Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp [[Part:BBa_K260002|]]. | + | Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp [[Part:BBa_K260002|BBa_K260002]]. |
===References=== | ===References=== |
Latest revision as of 08:07, 18 October 2009
pMA-RQ-@TetFlp
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2578
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2584 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2578
Illegal XhoI site found at 61
Illegal XhoI site found at 2572 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2578
Illegal suffix found at 2
Illegal XbaI site found at 67 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2578
Plasmid lacks a suffix.
Illegal XbaI site found at 67
Illegal XbaI site found at 2593
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
The 2x 70 bp homologies were designed to allow "pseudo-cloning" of a BioBrick from the present backbone pMA-RQ-@TetFlp to pTetFlp BBa_K260002 via recombineering using the PvuII fragment containing the BioBrick, where BioBrick prefix and suffix are kept untouched.
Source
Geneart plasmid pMA-RQ, with 2x 70 bp from pTetFlp BBa_K260002.