Difference between revisions of "Part:BBa K260004:Design"
 
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===Design Notes===
 
===Design Notes===
 
The 2x 70 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|BBa_K260001]]-[[Part:BBa_K260016|BBa_K260016]] and put the FRT site of [[Part:BBa_K260015|BBa_K260015]] (if used with the present backbone '''BBa_K260004''') at a defined distance from the FRT site of [[Part:BBa_K260016|BBa_K260016]].
 
The 2x 70 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|BBa_K260001]]-[[Part:BBa_K260016|BBa_K260016]] and put the FRT site of [[Part:BBa_K260015|BBa_K260015]] (if used with the present backbone '''BBa_K260004''') at a defined distance from the FRT site of [[Part:BBa_K260016|BBa_K260016]].
 +
  
 
===Source===
 
===Source===
  
−
Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB [[Part:K260001|K260001]].
+
Geneart plasmid pMA, with 2x 70 bp from pCC2FosMB [[Part:K260001|K260001]].
 +
 
  
 
===References===
 
===References===

Latest revision as of 07:33, 18 October 2009

pMA-@CC2FosMB-00500bp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2544
    Illegal NheI site found at 52
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2544
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2544
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2544
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2559
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The 2x 70 bp homologies were designed to delete 100 bp of BBa_K260001-BBa_K260016 and put the FRT site of BBa_K260015 (if used with the present backbone BBa_K260004) at a defined distance from the FRT site of BBa_K260016.


Source

Geneart plasmid pMA, with 2x 70 bp from pCC2FosMB K260001.


References