Difference between revisions of "Part:BBa K4882002"
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− | + | The Mcl1 signal peptide (Mcl1ss) is from the collagen-like protein MCL1 of M. anisopliae. Mcl1ss is an 18-amino acid secretory signal peptide at the N-terminus of the MCL1 protein (Wang & St Leger, 2006). | |
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<partinfo>BBa_K4882002 parameters</partinfo> | <partinfo>BBa_K4882002 parameters</partinfo> | ||
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+ | <!-- Add more about the biology of this part here--> | ||
+ | ===Usage and Biology=== | ||
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+ | To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene. | ||
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+ | ==References== | ||
+ | Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6647-52. doi: 10.1073/pnas.0601951103. Epub 2006 Apr 13. PMID: 16614065; PMCID: PMC1458935. |
Latest revision as of 14:48, 4 October 2023
MCL1 signal peptide from Metarhizium anisopliae
The Mcl1 signal peptide (Mcl1ss) is from the collagen-like protein MCL1 of M. anisopliae. Mcl1ss is an 18-amino acid secretory signal peptide at the N-terminus of the MCL1 protein (Wang & St Leger, 2006).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
To improve the efficiency of M. anisopliae as a fungal biopesticide, we choose to introduce the LqhIT2 toxin into the fungus. To promote the secretion of the toxin, a Mcl1ss is linked at the 5’ end of the LqhIT2 gene.
References
Wang C, St Leger RJ. A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6647-52. doi: 10.1073/pnas.0601951103. Epub 2006 Apr 13. PMID: 16614065; PMCID: PMC1458935.