Difference between revisions of "Part:BBa K260007:Design"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K260007 short</partinfo>
 
<partinfo>BBa_K260007 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The 50 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|]]-[[Part:BBa_K260016|]] and put the FRT site of [[Part:BBa_K260015|]] (if used with the present backbone [[Part:BBa_K260007|]]) at a defined distance from the FRT site of [[Part:BBa_K260016|]].
+
The 2x 70 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|BBa_K260001]]-[[Part:BBa_K260016|BBa_K260016]] and put the FRT site of [[Part:BBa_K260015|BBa_K260015]] (if used with the present backbone '''BBa_K260007''') at a defined distance from the FRT site of [[Part:BBa_K260016|BBa_K260016]].
  
  
Line 13: Line 12:
 
===Source===
 
===Source===
  
−
Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB [[Part:K260001|]].
+
Geneart plasmid pMA, with 2x 70 bp from pCC2FosMB [[Part:K260001|K260001]].
 +
 
  
 
===References===
 
===References===

Latest revision as of 07:33, 18 October 2009

pMA-@CC2FosMB-05000bp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2544
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2544
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2544
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2544
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2559
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The 2x 70 bp homologies were designed to delete 100 bp of BBa_K260001-BBa_K260016 and put the FRT site of BBa_K260015 (if used with the present backbone BBa_K260007) at a defined distance from the FRT site of BBa_K260016.


Source

Geneart plasmid pMA, with 2x 70 bp from pCC2FosMB K260001.


References