Difference between revisions of "Part:BBa K260004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The 70 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|BBa_K260001]]-[[Part:BBa_K260016|BBa_K260016]] and put the FRT site of [[Part:BBa_K260015|BBa_K260015]] (if used with the present backbone '''BBa_K260004''') at a defined distance from the FRT site of [[Part:BBa_K260016|BBa_K260016]]. | + | The 2x 70 bp homologies were designed to delete 100 bp of [[Part:BBa_K260001|BBa_K260001]]-[[Part:BBa_K260016|BBa_K260016]] and put the FRT site of [[Part:BBa_K260015|BBa_K260015]] (if used with the present backbone '''BBa_K260004''') at a defined distance from the FRT site of [[Part:BBa_K260016|BBa_K260016]]. |
===Source=== | ===Source=== | ||
Revision as of 07:28, 18 October 2009
pMA-@CC2FosMB-00500bp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2544
Illegal NheI site found at 52
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2550 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2544 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2544
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2544
Plasmid lacks a suffix.
Illegal XbaI site found at 2559
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
The 2x 70 bp homologies were designed to delete 100 bp of BBa_K260001-BBa_K260016 and put the FRT site of BBa_K260015 (if used with the present backbone BBa_K260004) at a defined distance from the FRT site of BBa_K260016.
Source
Geneart plasmid pMA, with 2x 50 bp from pCC2FosMB K260001.