Difference between revisions of "Part:BBa K4790048"

 
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4790048 short</partinfo>
 
<partinfo>BBa_K4790048 short</partinfo>
  
−
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding alanine.
+
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding histidine.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:13, 4 October 2023


ASR1-N81H-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 81st amino acid from aspartic acid to histidine. According to Codon preference in E.coli, CAT has the highest possibility coding histidine.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]