Difference between revisions of "Part:BBa K4790046"
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<partinfo>BBa_K4790046 short</partinfo> | <partinfo>BBa_K4790046 short</partinfo> | ||
| − | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on | + | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 79th amino acid from glutamine to aspartic acid. According to Codon preference in E.coli, AAT has the highest possibility coding aspartic acid. |
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Latest revision as of 12:12, 4 October 2023
ASR1-Q79N-F
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 79th amino acid from glutamine to aspartic acid. According to Codon preference in E.coli, AAT has the highest possibility coding aspartic acid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 19
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 19
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 19
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 19
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 19
- 1000COMPATIBLE WITH RFC[1000]