Difference between revisions of "Part:BBa K4790046"

 
 
(One intermediate revision by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K4790046 short</partinfo>
 
<partinfo>BBa_K4790046 short</partinfo>
  
−
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 79th amino acid from glutamine to aspartic acid. According to Codon preference in E.coli, AAT has the highest possibility coding alanine.
+
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 79th amino acid from glutamine to aspartic acid. According to Codon preference in E.coli, AAT has the highest possibility coding aspartic acid.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:12, 4 October 2023


ASR1-Q79N-F

The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on node 59 and coding the 79th amino acid from glutamine to aspartic acid. According to Codon preference in E.coli, AAT has the highest possibility coding aspartic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 19
  • 1000
    COMPATIBLE WITH RFC[1000]