Difference between revisions of "Part:BBa K4800001"
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− | + | <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/mmcar.png" width="60%" height="60%"></p> | |
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− | <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/mmcar.png" width=" | + | |
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− | <strong>Fig. | + | <strong>Fig.1 Catalytic pocket for MmCAR</strong> |
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<p>MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino | <p>MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino | ||
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the original one | the original one | ||
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− | + | <p style="font-size: 160%; font-weight: bold;">Result: | |
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+ | <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/q302e.png" width="80%" height="80%"></p> | ||
+ | <div align="center"> | ||
+ | <strong>Fig.2 Q302E and MmCAR produce 1,5-PDO in KA30</strong> | ||
+ | </div> | ||
+ | <p>Through fermentation experiments, we have verified that Q302E can produce more pentanediol | ||
+ | </p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
− | <partinfo> | + | <partinfo>BBa_K4800001 SequenceAndFeatures</partinfo> |
Revision as of 11:45, 4 October 2023
rrnB T1 terminator
Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)
Fig.1 Catalytic pocket for MmCAR
MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one
Result:
Fig.2 Q302E and MmCAR produce 1,5-PDO in KA30
Through fermentation experiments, we have verified that Q302E can produce more pentanediol
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]