Difference between revisions of "Part:BBa K4800001"
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promoter vgb fine tunes the inhibitory effect of oxygen on the promoter. | promoter vgb fine tunes the inhibitory effect of oxygen on the promoter. | ||
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− | <p><img src="https://static.igem.wiki/teams/ | + | <p><img src="https://static.igem.wiki/teams/4800/wiki/parts/mmcar.png" width="80%" height="80%"></p> |
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<strong>Fig.9 Construction of the recombinant plasmid for pMTL-PvgbF7-bs2</strong> | <strong>Fig.9 Construction of the recombinant plasmid for pMTL-PvgbF7-bs2</strong> | ||
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− | <p> | + | <p>MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino |
− | + | acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to | |
− | + | the original one | |
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Revision as of 11:38, 4 October 2023
rrnB T1 terminator
Carboxylate reductase from Mycobacterium marinum(Mutation of glutamine at position 302 to glutamate)
Result:
Adjusting the distance between FNR binding site and the -35 region of promoter vgb fine tunes the inhibitory effect of oxygen on the promoter.
Fig.9 Construction of the recombinant plasmid for pMTL-PvgbF7-bs2
MmCAR is a carboxylic acid reductase from marine bacteria. During the experiment, we mutated the original 302nd amino acid glutamine to glutamic acid through acid-base mutation. The mutated Q302E has a significant improvement compared to the original one
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 357
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 357
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 357
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 357
- 1000COMPATIBLE WITH RFC[1000]