Difference between revisions of "Part:BBa K4767002"
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<partinfo>BBa_K4767002 short</partinfo> | <partinfo>BBa_K4767002 short</partinfo> | ||
| − | This part encodes a tyrosine phosphatase involved in the biosynthesis of extracellular polysaccharide in Shewanella oneidensis MR-1. Unconductive polysaccharide in S. oneidensis MR-1biofilm matrix attenuate the efficiency of extracellular electron transfer. We knocked out ptpA from S. oneidensis MR-1 genome to enhance its ability to generate electricity. | + | This part encodes a tyrosine phosphatase involved in the biosynthesis of extracellular polysaccharide in <i>Shewanella oneidensis</i> MR-1. Unconductive polysaccharide in <i>S. oneidensis</i> MR-1biofilm matrix attenuate the efficiency of extracellular electron transfer. We knocked out <i>ptpA</i> from <i>S. oneidensis</i> MR-1 genome to enhance its ability to generate electricity. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | We found that <i>ptpA</i> gene in <i>S. oneidensis</i> MR-1 is highly homologous with the WzB in the <i>Escherichia coli</i>. In<i> E. coli</i>, WzB plays an important role in polysaccharide biosynthesis by acting as a protein-tyrosine phosphatase. By knocking out <i>ptpA</i> gene and complementation, we verified that <i>ptpA</i> gene in <i>S. oneidensis</i> MR-1may possibly effect the biofilm formation and its conductivity by meditating the polysaccharide biosynthesis. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 10:04, 4 October 2023
ptpA
This part encodes a tyrosine phosphatase involved in the biosynthesis of extracellular polysaccharide in Shewanella oneidensis MR-1. Unconductive polysaccharide in S. oneidensis MR-1biofilm matrix attenuate the efficiency of extracellular electron transfer. We knocked out ptpA from S. oneidensis MR-1 genome to enhance its ability to generate electricity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]