Difference between revisions of "Part:BBa B0014:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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This review comes from the old result system and indicates that this part worked in some test. | This review comes from the old result system and indicates that this part worked in some test. | ||
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| + | <partinfo>BBa_B0014 AddReview 5</partinfo> | ||
| + | <I>iGEM Groningen 2009</I> | ||
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| + | The plasmid containing part <partinfo>BBa_B0014</partinfo> was successfully transformed into ''E. coli'' TOP10 cells (confirmed by single and double digestion). The original plan was to use the terminator behind one of our own designed parts <partinfo>BBa_K190027</partinfo>, and in front of pArsR-RFP. The MBP-ArsR fusion protein was thought to have a regulating effect on the arsenic promotor pArsR in the same way as ArsR regulates the promotor. The terminator separated the two parts on the plasmid. The construct was not used due to time constraints, and not sequenced. | ||
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<!-- DON'T DELETE --><partinfo>BBa_B0014 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_B0014 EndReviews</partinfo> | ||
Revision as of 06:23, 18 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_B0014
User Reviews
UNIQ44bcaa19fcc68c22-partinfo-00000000-QINU
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•••••
Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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•••••
iGEM Groningen 2009 |
The plasmid containing part BBa_B0014 was successfully transformed into E. coli TOP10 cells (confirmed by single and double digestion). The original plan was to use the terminator behind one of our own designed parts BBa_K190027, and in front of pArsR-RFP. The MBP-ArsR fusion protein was thought to have a regulating effect on the arsenic promotor pArsR in the same way as ArsR regulates the promotor. The terminator separated the two parts on the plasmid. The construct was not used due to time constraints, and not sequenced. |
UNIQ44bcaa19fcc68c22-partinfo-00000005-QINU