Difference between revisions of "Part:BBa K4634007"

 
 
Line 9: Line 9:
  
 
<!-- -->
 
<!-- -->
−
<span class='h3bb'>Sequence and Features</span>
+
===Sequence and Features===
 +
<span class='h3bb'></span>
 
<partinfo>BBa_K4634007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4634007 SequenceAndFeatures</partinfo>
  
Line 17: Line 18:
 
<partinfo>BBa_K4634007 parameters</partinfo>
 
<partinfo>BBa_K4634007 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. 10.1128/AEM.04023-14 PubMed 25636838

Latest revision as of 05:15, 4 October 2023


pTargetF with gRNA designed

PTargetF is one of the commonly used plasmids in E. coli gene knockout. Mainly including sgRNA expression elements, used to load repair templates and sgRNA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 198
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 342
    Illegal BamHI site found at 186
    Illegal XhoI site found at 366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1137
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 122


References

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system. Jiang Y, Chen B, Duan C, Sun B, Yang J, Yang S. Appl Environ Microbiol. 2015 Jan 30. pii: AEM.04023-14. 10.1128/AEM.04023-14 PubMed 25636838