Difference between revisions of "Part:BBa K4634001"

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__NOTOC__
 
__NOTOC__
<partinfo>BBa_K4634001 short</partinfo>
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<partinfo>BBa_K4634002 short</partinfo>
  
 
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989).
 
efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989).
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
<partinfo>BBa_K4634001 SequenceAndFeatures</partinfo>
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<span class='h3bb'></span>
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<partinfo>BBa_K4634002 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
<partinfo>BBa_K4634001 parameters</partinfo>
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<partinfo>BBa_K4634002 parameters</partinfo>
 
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Revision as of 04:23, 4 October 2023


pET28a(+) backbone

efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5242
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 5242
    Illegal BglII site found at 5130
    Illegal XhoI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 5242
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 5242
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5257
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 3350
    Illegal NgoMIV site found at 3510
    Illegal NgoMIV site found at 5098
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2429