Difference between revisions of "Part:BBa K4897005"

Revision as of 02:54, 4 October 2023

Worm L-RCA (BS DNA 4.0) for detecting Myotonic Dystrophy

Our collaborators are very informative and interested in our researches in L-RCA. After they successfully replicated our results, they trust in our quality and creativity and ask us for novel detection or diagnosis of certain human diseases.One of the disease is called Myotonic Dystrophy which is hard to diagnose in current stage. Here is the general description. Myotonic Dystrophy has different types according to its severity which is based on the amount of repetitions of 5’ CTG 3’[2]. Myotonic Dystrophy Type 1 (DM1) is more severe and has more repetitions than Myotonic Dystrophy Type 2 (DM2). Due to the variability of the disease gene, the current method is flawed in its accuracy and time duration. However, Worm L-RCA is able to solve this issue. It has two DNA fragments working: BS Worm DNA-300 and BS Worm DNA-12. BS Worm DNA-300 is designed and is similar to previous DNAs which bind to P. acne’s DNA. It has the following composition: binding region, amplification region, and random region.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 218
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 218
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 218
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 218
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 255
Illegal BsaI.rc site found at 13

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 <partinfo>BBa_K4897005 short</partinfo>
-Our collaborators are very informative and interested in our researches in L-RCA. After they successfully replicated our results, they trust in our quality and creativity and ask us for novel detection or diagnosis of certain human diseases.
+Our collaborators are very informative and interested in our researches in L-RCA. After they successfully replicated our results, they trust in our quality and creativity and ask us for novel detection or diagnosis of certain human diseases.One of the disease is called Myotonic Dystrophy which is hard to diagnose in current stage. Here is the general description. Myotonic Dystrophy has different types according to its severity which is based on the amount of repetitions of 5’ CTG 3’[2]. Myotonic Dystrophy Type 1 (DM1) is more severe and has more repetitions than Myotonic Dystrophy Type 2 (DM2). Due to the variability of the disease gene, the current method is flawed in its accuracy and time duration. However, Worm L-RCA is able to solve this issue. It has two DNA fragments working: BS Worm DNA-300 and BS Worm DNA-12. BS Worm DNA-300 is designed and is similar to previous DNAs which bind to P. acne’s DNA. It has the following composition: binding region, amplification region, and random region.
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