Difference between revisions of "Part:BBa K4593002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sequence is codon optimized for expression in E. coli. | |
===Source=== | ===Source=== | ||
Revision as of 17:34, 3 October 2023
ClyC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 681
Illegal AgeI site found at 550 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is codon optimized for expression in E. coli.
Source
Hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12.
References
Lee, Chanyoung, et al. “Development of Advanced Chimeric Endolysin to Control Multidrug-Resistant Staphylococcus Aureus through Domain Shuffling.” ACS Infectious Diseases, vol. 7, no. 8, 28 May 2021, pp. 2081–2092. https://doi.org/10.1021/acsinfecdis.0c00812.