Difference between revisions of "Part:BBa K4839019"
| Line 9: | Line 9: | ||
Promoters like HPGK contain specific DNA sequences recognized by RNA polymerase, an enzyme responsible for initiating transcription. When RNA polymerase binds to the HPGK promoter, it begins the process of copying the PGK gene into mRNA. This mRNA is then translated into the PGK enzyme, which facilitates the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate in glycolysis, a crucial step for energy production in cells. | Promoters like HPGK contain specific DNA sequences recognized by RNA polymerase, an enzyme responsible for initiating transcription. When RNA polymerase binds to the HPGK promoter, it begins the process of copying the PGK gene into mRNA. This mRNA is then translated into the PGK enzyme, which facilitates the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate in glycolysis, a crucial step for energy production in cells. | ||
| − | In our experiment, we use hPGK promote to initiate the exxpression of several proteins, including Anti-GPC3 SNIPR and IRF4-GFP. | + | <p>In our experiment, we use hPGK promote to initiate the exxpression of several proteins, including Anti-GPC3 SNIPR and IRF4-GFP. </p> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:22, 3 October 2023
hPGK promoter
The HPGK promoter, short for Human Phosphoglycerate Kinase promoter, is a critical regulatory region in the human genome. It controls the initiation of gene expression for the phosphoglycerate kinase (PGK) gene, specifically in humans. The PGK enzyme plays a vital role in glycolysis, a metabolic pathway essential for energy production within cells.
The HPGK promoter is essential for ensuring the accurate and timely transcription of the PGK gene into messenger RNA (mRNA). This mRNA serves as a template for protein synthesis, ultimately leading to the production of the PGK enzyme.
Promoters like HPGK contain specific DNA sequences recognized by RNA polymerase, an enzyme responsible for initiating transcription. When RNA polymerase binds to the HPGK promoter, it begins the process of copying the PGK gene into mRNA. This mRNA is then translated into the PGK enzyme, which facilitates the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate in glycolysis, a crucial step for energy production in cells.
In our experiment, we use hPGK promote to initiate the exxpression of several proteins, including Anti-GPC3 SNIPR and IRF4-GFP.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 739
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 739
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 739
- 1000COMPATIBLE WITH RFC[1000]