Difference between revisions of "Part:BBa K4814014"

Revision as of 12:19, 3 October 2023

RecA(K6)-B0034-eGFP

Hoping to validate previous iGEM team’s result and investigate the impact of the genotoxicity of carcinogens, we conducted dosage dependent tests on the top10 E.coli bacteria. RecA(K3), used with eGFP (BBa_K302002) as bioreporter to detect DNA damaging agents such as UV, H2O2 and nalidixic acid, is the promoter derived from BBa_K3020001 by team BIT 2019. This promoter is an optimized version of BBa_K629001 (K6), which is a design of team SYSU 2011 aimed to drive the motor system under a radioactive environment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 881
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 839
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K4814014 short</partinfo>
-Hoping to validate previous iGEM team’s result and investigate the impact of the genotoxicity of carcinogens, we conducted dosage dependent tests on the top10 E.coli bacteria. RecA(K3), used with eGFP (composite part BBa_K3020000) as bioreporter to detect DNA damaging agents such as UV, H2O2 and nalidixic acid, is the promoter derived from BBa_K3020001 by team BIT 2019. This promoter is BBa_K629001 (K6), a design of team SYSU 2011 aimed to drive the motor system under a radioactive environment. We design this composite part using promoter BBa_K629001, RBS BBa_B0034, and eGFP BBa_K3020002.
+Hoping to validate previous iGEM team’s result and investigate the impact of the genotoxicity of carcinogens, we conducted dosage dependent tests on the top10 E.coli bacteria. RecA(K3), used with eGFP (BBa_K302002) as bioreporter to detect DNA damaging agents such as UV, H2O2 and nalidixic acid, is the promoter derived from BBa_K3020001 by team BIT 2019. This promoter is an optimized version of BBa_K629001 (K6), which is a design of team SYSU 2011 aimed to drive the motor system under a radioactive environment.
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