Difference between revisions of "Part:BBa K4937030"

 
 
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<partinfo>BBa_K4937030 short</partinfo>
 
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<p>This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.</p>
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https://static.igem.wiki/teams/4937/wiki/part/24-1.png
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<p>We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of <i>S. cerevisiae</i> and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2). </p>
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https://static.igem.wiki/teams/4937/wiki/part/24-2.png
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https://static.igem.wiki/teams/4937/wiki/part/24-3.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 11:30, 3 October 2023


TEF1p-AtACL5-CYC1t

This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.

24-1.png

We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of S. cerevisiae and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2).

24-2.png 24-3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167