Difference between revisions of "Part:BBa K4937025"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4937025 short</partinfo> | <partinfo>BBa_K4937025 short</partinfo> | ||
| + | <p>This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.</p> | ||
| + | https://static.igem.wiki/teams/4937/wiki/part/24-1.png | ||
| + | <p>We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of <i>S. cerevisiae</i> and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2). </p> | ||
| + | https://static.igem.wiki/teams/4937/wiki/part/24-2.png | ||
| + | https://static.igem.wiki/teams/4937/wiki/part/24-3.png | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 11:28, 3 October 2023
TEF1p-SPE3-PRM9t
This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.
We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of S. cerevisiae and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1346
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 167