Difference between revisions of "Part:BBa K4937024"
 
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<partinfo>BBa_K4937024 short</partinfo>
 
<partinfo>BBa_K4937024 short</partinfo>
  
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<p>This part was used to constitutively express SPE2, and it was integrated with BBa_K4937025/BBa_K4937026/BBa_K4937027/BBa_K4937028/BBa_K4937029 or BBa_K4937030/BBa_K4937031/BBa_K4937032/BBa_K4937033 to enhance the flux of polyamine synthesis in <i>Saccharomyces cerevisiae</i>, and also the product of thermospermine, which may confer thermotolerance to strain.</p>
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<p>This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.</p>
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https://static.igem.wiki/teams/4937/wiki/part/24-1.png
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<p>We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of <i>S. cerevisiae</i> and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2). </p>
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https://static.igem.wiki/teams/4937/wiki/part/24-2.png
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https://static.igem.wiki/teams/4937/wiki/part/24-3.png
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
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<partinfo>BBa_K4937024 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4937024 SequenceAndFeatures</partinfo>
  

Latest revision as of 11:27, 3 October 2023


TDH3p-SPE2-DIT1t

This part is used to construct the plasmid library that can stably express SPE2 and tune the expression level of SPE3 and AtACL5 to fully use the spermidine substrate and examine the numerical relationship between spermidine and thermospermine.

24-1.png

We constructed this part by two round PCR. We firstly achieved all short single fragment by PCR from the genome of S. cerevisiae and plasmid (Figure 1). Then we connected all single expression device by fusion PCR (Figure 2).

24-2.png 24-3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]