Difference between revisions of "Part:BBa K4765129"

 
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<partinfo>BBa_K4765129 short</partinfo>
 
<partinfo>BBa_K4765129 short</partinfo>
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html>
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__TOC__
  
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stem-loop test
 
  
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<!-- Add more about the biology of this part here
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===Introduction===
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===Usage and Biology===
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This compoiste part is a stem-loop-deleted version of [https://parts.igem.org/Part:BBa_K4765120 BBa_K4765120( ribozyme test: T7 leaky expression)], which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with [something] to assess the stem-loop's ability to prevent mRNA degradation.
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===Characterization===
  
 
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Revision as of 08:00, 2 October 2023


stem-loop test contributed by Fudan iGEM 2023


Introduction

This compoiste part is a stem-loop-deleted version of BBa_K4765120( ribozyme test: T7 leaky expression), which includes StayGold and mScarlet. Its red-green fluorescence intensity ratio can be compared with [something] to assess the stem-loop's ability to prevent mRNA degradation.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1389
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 720