Difference between revisions of "Part:BBa K4765112"
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This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in [https://parts.igem.org/Part:BBa_K2306003 Secretory-abundant heat soluble protein 33020 (SAHS 33020)]. | This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in [https://parts.igem.org/Part:BBa_K2306003 Secretory-abundant heat soluble protein 33020 (SAHS 33020)]. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We constructed SAHS 33020 into pRAP to endow ''E. coli'' with desiccation-resistant capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765113 ribozyme+T7_RBS+mtSSB+stem-loop] to increase the survival rate of ''E.coli'' under desiccation. | + | We constructed SAHS 33020 into pRAP to endow ''E. coli'' with desiccation-resistant capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765113 BBa_K4765113(ribozyme+T7_RBS+mtSSB+stem-loop)] to increase the survival rate of ''E.coli'' under desiccation. |
===Characterization=== | ===Characterization=== | ||
Revision as of 07:57, 2 October 2023
Twister P1 + T7_RBS + SAHS 33020 + stem-loop
Introduction
This composite part is composed of Secretory-abundant heat soluble protein 33020 (SAHS 33020) and ribozyme-assisted polycistronic co-expression system：pRAP. SAHS 33020 is a heat-soluble protein in tardigrades that helps them survive tough conditions by forming a protective glass matrix during desiccation. It was tested by iGEM17_TUDelft in Secretory-abundant heat soluble protein 33020 (SAHS 33020).
Usage and Biology
We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant capability. And this composite part can cooperate with BBa_K4765113(ribozyme+T7_RBS+mtSSB+stem-loop) to increase the survival rate of E.coli under desiccation.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 178