Difference between revisions of "Part:BBa K4765126"
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Siliang Zhan (Talk | contribs) |
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===Introduction=== | ===Introduction=== | ||
This composite part combines [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)] in our ribozyme-assisted polycistronic co-expression system:pRAP. | This composite part combines [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)] in our ribozyme-assisted polycistronic co-expression system:pRAP. | ||
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AnAFP is an antifreeze protein which endows ''E. coli'' with antifreeze capibility. SAHS 33020 is a heat soluble protein found in the tardigrades which increase ''E. coli'' 's stability against desiccation. ''H. ex'' mtSSB is a type of mitochondrial single-stranded DNA binding protein which improves ''E. coli'' 's resistance to desiccation and UV radiation. | AnAFP is an antifreeze protein which endows ''E. coli'' with antifreeze capibility. SAHS 33020 is a heat soluble protein found in the tardigrades which increase ''E. coli'' 's stability against desiccation. ''H. ex'' mtSSB is a type of mitochondrial single-stranded DNA binding protein which improves ''E. coli'' 's resistance to desiccation and UV radiation. | ||
Revision as of 07:11, 2 October 2023
ribozyme connected: H. ex mtSSB + SAHS 33020 + AnAFP
Contents
Introduction
This composite part combines BBa_K4765016(H. ex mtSSB),BBa_K2306003(SAHS 33020)and BBa_K4765015(AnAFP) in our ribozyme-assisted polycistronic co-expression system:pRAP.
AnAFP is an antifreeze protein which endows E. coli with antifreeze capibility. SAHS 33020 is a heat soluble protein found in the tardigrades which increase E. coli 's stability against desiccation. H. ex mtSSB is a type of mitochondrial single-stranded DNA binding protein which improves E. coli 's resistance to desiccation and UV radiation.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 247
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 880
Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409
Illegal SapI.rc site found at 1804