Difference between revisions of "Part:BBa K4765126"

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===Usage and Biology===
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===Introduction===
 
This composite part combines [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)] in our ribozyme-assisted polycistronic co-expression system：pRAP.
 
This composite part combines [https://parts.igem.org/Part:BBa_K4765016 BBa_K4765016(''H. ex'' mtSSB)],[https://parts.igem.org/Part:BBa_K2306003 BBa_K2306003(SAHS 33020)]and [https://parts.igem.org/Part:BBa_K4765015 BBa_K4765015(AnAFP)] in our ribozyme-assisted polycistronic co-expression system：pRAP.
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AnAFP is an antifreeze protein which endows ''E. coli'' with antifreeze capibility. SAHS 33020 is a heat soluble protein found in the tardigrades which increase ''E. coli'''s stability against desiccation. ''H. ex'' mtSSB is a type of mitochondrial single-stranded DNA binding protein which improves ''E. coli'''s resistance to desiccation and UV radiation.
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===Characterization===
 
===Characterization===
  

Revision as of 07:10, 2 October 2023


ribozyme connected: H. ex mtSSB + SAHS 33020 + AnAFP contributed by Fudan iGEM 2023


Introduction

This composite part combines BBa_K4765016(H. ex mtSSB),BBa_K2306003(SAHS 33020)and BBa_K4765015(AnAFP) in our ribozyme-assisted polycistronic co-expression system：pRAP. AnAFP is an antifreeze protein which endows E. coli with antifreeze capibility. SAHS 33020 is a heat soluble protein found in the tardigrades which increase E. colis stability against desiccation. H. ex mtSSB is a type of mitochondrial single-stranded DNA binding protein which improves E. colis resistance to desiccation and UV radiation.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 880
    Illegal BsaI.rc site found at 515
    Illegal BsaI.rc site found at 596
    Illegal SapI site found at 409
    Illegal SapI.rc site found at 1804