Difference between revisions of "Part:BBa K4765014"
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| − | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273014 BBa_K4273014 | + | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273014 BBa_K4273014(NlmysH)] specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''. |
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==References== | ==References== | ||
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Revision as of 07:07, 2 October 2023
MysH codon optimized
Introduction
Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs[1].
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| Figure1 The biosynthetic pathway of shinorine, porphyra-334, palythine-Ser, and palythine-Thr. 1 |
Usage and Biology
We performed codon optimization on BBa_K4273014(NlmysH) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 23
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.