Difference between revisions of "Part:BBa K4621020"

 
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__NOTOC__
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<partinfo>BBa_K4621020 short</partinfo>
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Usage, Biology and Characterization：
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gadB is the coding sequence for glutamate decarboxylase, GAD is a pyridoxal 5'phosphate (PLP)-dependent decarboxylase widespread in bacteria yeast and filamentous fungi. The carboxyl group (-COOH) on the catalytic glutamate molecule is released to CO2, and 4-Aminobutanoate is generated at the same time, which participates in the utilization of α-Ketoglutaricacid in the TCA cycle. The specific synthesis route is shown in FIG. 1.
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Fig.1 Biosynthetic pathway of GABA
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Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows:
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[WT GABA Gi1 3 9 fermentation data graph]
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4621020 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4621020 parameters</partinfo>
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Revision as of 17:22, 1 October 2023


gadB is the coding sequence of glutamate decarboxylase, which converts glutamate to 4-Aminobutanoate

Usage, Biology and Characterization：

gadB is the coding sequence for glutamate decarboxylase, GAD is a pyridoxal 5'phosphate (PLP)-dependent decarboxylase widespread in bacteria yeast and filamentous fungi. The carboxyl group (-COOH) on the catalytic glutamate molecule is released to CO2, and 4-Aminobutanoate is generated at the same time, which participates in the utilization of α-Ketoglutaricacid in the TCA cycle. The specific synthesis route is shown in FIG. 1.

Fig.1 Biosynthetic pathway of GABA


Modified Streptomyces SCUT-3. They were then inoculated in a 40 mL LB medium system. After fermentation at 40 ° C and 220 rpm for 72 hours, the product GABA was measured, and the results were as follows: [WT GABA Gi1 3 9 fermentation data graph]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 53
    Illegal NgoMIV site found at 1242
    Illegal AgeI site found at 470
    Illegal AgeI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]