Difference between revisions of "Part:BBa K4765120"
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<html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | ||
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Revision as of 17:19, 1 October 2023
ribozyme test: T7 leaky expression
Introduction
This composite part is utilized to assess the cleavage efficiency of Twister P1 and stem-loop’s ability of preventing mRNA degradation. It is regulated by T7 promoter ,lac operator and T7 terminator. From its upstream to understream includes StayGold, stem-loop, Twister P1 and mScarlet. A ribozyme is proved to have cleavage ability when green and red fluorescence are emitted at the same time. We can assess the cleavage efficiency of ribozyme based on the ratio of red-green fluorescence intensity when the stem-loop is unchanged. We can also assess stem-loop’s ability of preventing mRNA degradation based on the ratio of red-green fluorescence intensity when the ribozyme is unchanged.
Usage and Biology
This composite part is an easy and effective tool to select the fit ribozyme and stem-loop.
characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1409
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 700
Illegal BsaI.rc site found at 720