Difference between revisions of "Part:BBa K4765120"

 
Line 3: Line 3:
 
<partinfo>BBa_K4765120 short</partinfo>
 
<partinfo>BBa_K4765120 short</partinfo>
  
−
ribozyme measurement module: Twister P1 (constitutive expression)
+
===Introduction===
 +
This composite part is utilized to assess the cleavage efficiency of Twister P1 and stem-loop’s ability of preventing mRNA degradation. It is regulated by T7 promoter ,lac operator and T7 terminator. From its upstream to understream includes StayGold, stem-loop, Twister P1 and mScarlet.
 +
A ribozyme is proved to have cleavage ability when green and red fluorescence are emitted at the same time. We can assess the cleavage efficiency of ribozyme based on the ratio of red-green fluorescence intensity when the stem-loop is unchanged. We can also assess stem-loop’s ability of preventing mRNA degradation based on the ratio of red-green fluorescence intensity when the ribozyme is unchanged.
 +
===Usage and Biology===
 +
This composite part is an easy and effective tool to select the fit ribozyme and stem-loop.
 +
===characterization==
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:18, 1 October 2023


ribozyme test: T7 leaky expression

Introduction

This composite part is utilized to assess the cleavage efficiency of Twister P1 and stem-loop’s ability of preventing mRNA degradation. It is regulated by T7 promoter ,lac operator and T7 terminator. From its upstream to understream includes StayGold, stem-loop, Twister P1 and mScarlet. A ribozyme is proved to have cleavage ability when green and red fluorescence are emitted at the same time. We can assess the cleavage efficiency of ribozyme based on the ratio of red-green fluorescence intensity when the stem-loop is unchanged. We can also assess stem-loop’s ability of preventing mRNA degradation based on the ratio of red-green fluorescence intensity when the ribozyme is unchanged.

Usage and Biology

This composite part is an easy and effective tool to select the fit ribozyme and stem-loop.

=characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1409
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 700
    Illegal BsaI.rc site found at 720