Difference between revisions of "Part:BBa K4765115"
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===Introduction===
 
===Introduction===

Revision as of 12:45, 1 October 2023


Twister P1 + T7_RBS + FEN1 + stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging "flaps" formed when DNA strands can't bind correctly.

Usage and Biology

We constructed FEN1into pRAP to endow ‘’E. coli’’ with anti-UV capability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
    Illegal AgeI site found at 301
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference