Difference between revisions of "Part:BBa K4765106"
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<partinfo>BBa_K4765106 short</partinfo> | <partinfo>BBa_K4765106 short</partinfo> | ||
| + | <html><img style="float:right;width:128px" src="https://static.igem.wiki/teams/4765/wiki/2023-b-home.png" alt="contributed by Fudan iGEM 2023"></html> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Similar to [https://parts.igem.org/Part:BBa_K4765104 ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop], we replaced Nb2 with Nb3 in order to interact with [https://parts.igem.org/Part:BBa_K4765105 ribozyme+strong RBS+intimin-Ag3 fusion+stem-loop] to create a biofilm. | Similar to [https://parts.igem.org/Part:BBa_K4765104 ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop], we replaced Nb2 with Nb3 in order to interact with [https://parts.igem.org/Part:BBa_K4765105 ribozyme+strong RBS+intimin-Ag3 fusion+stem-loop] to create a biofilm. | ||
Revision as of 12:40, 1 October 2023
Twister P1 + T7_RBS + intimin-Nb3 fusion + stem-loop
Contents
Usage and Biology
Similar to ribozyme+strong RBS+intimin-Nb2 fusion+stem-loop, we replaced Nb2 with Nb3 in order to interact with ribozyme+strong RBS+intimin-Ag3 fusion+stem-loop to create a biofilm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1305
Illegal BsaI.rc site found at 2201