Difference between revisions of "Part:BBa K4765015"
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===Introduction===
 
===Introduction===
 
''Ammopiptanthus nanus''antifreeze protein (AnAFP) is an antifreeze protein derived from ''Ammopiptanthus nanus''. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang,  S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of  antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold  tolerance in ''Escherichia coli'' and tobacco. ’‘Gene, 539’‘(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013</ref>.
 
''Ammopiptanthus nanus''antifreeze protein (AnAFP) is an antifreeze protein derived from ''Ammopiptanthus nanus''. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang,  S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of  antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold  tolerance in ''Escherichia coli'' and tobacco. ’‘Gene, 539’‘(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013</ref>.
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 12:25, 1 October 2023


AnAFP contributed by Fudan iGEM 2023

Introduction

Ammopiptanthus nanusantifreeze protein (AnAFP) is an antifreeze protein derived from Ammopiptanthus nanus. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions[1].


Usage and Biology

We heterologously expressed codon-optimized AnAFP in E.coli, endowing it with antifreeze capability.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 451


References

  1. ↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. ’‘Gene, 539’‘(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013