Difference between revisions of "Part:BBa K4765008"
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===Introduction=== | ===Introduction=== | ||
INPNC is a kind of modified INP with truncation of the entire internal repeating domain. INPNC does not appear to be hampered by the size of the passenger while also exhibiting compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers <ref>van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003 | INPNC is a kind of modified INP with truncation of the entire internal repeating domain. INPNC does not appear to be hampered by the size of the passenger while also exhibiting compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers <ref>van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003 | ||
Revision as of 11:47, 1 October 2023
INPNC
Introduction
INPNC is a kind of modified INP with truncation of the entire internal repeating domain. INPNC does not appear to be hampered by the size of the passenger while also exhibiting compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers [1]. A flexible protein domain linker of 10 aa was introduced between INPNC and protein sequence to ensure independent functionality of protein and INPNC with minimal mutual disruption
Usage and Biology
At the C-terminus of INPNC+linker, specific functional proteins can be attached to enable the surface display of it. In our project, we constructed K4765107 ribozyme+strong RBS+INPNC-Ag3 fusion+stem-loop and K4765108 ribozyme+strong RBS+INPNC-Nb3 fusion+stem-loop for the surface display of Ag/Nb2 and compared it with K4765105 ribozyme+strong RBS+intimin-Ag3 fusion+stem-loop、K4765106 ribozyme+strong RBS+intimin-Nb3 fusion+stem-loop. And selected intimin as our surface display system.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 481
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 405
Illegal AgeI site found at 429 - 1000COMPATIBLE WITH RFC[1000]
Reference
- ↑ van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003