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===Introduction===
 
===Introduction===

Revision as of 11:44, 1 October 2023


Nb1, 3AKH13 from 10.1016/j.cell.2018.06.041

contributed by Fudan iGEM 2023


Introduction

Nb1 is a variant of nanobody, a type of single-domain antibody[1].It can interact with Ag1and the interaction between Ag-Nb can mediate specific adhesion of Escherichia coli.

Usage and Biology

The combination of Nb1’s single-domain structure and the intimin surface display system allows the entirety of a highly specific, cell surface-bound adhesin to be encoded as a single fusion protein.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

  1. ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041