Difference between revisions of "Part:BBa K4765113"

Revision as of 08:20, 1 October 2023

Twister P1 + T7_RBS + H. ex mtSSB + stem-loop

Introduction

This composite part is composed of Hypsibius exemplaris mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and ribozyme-assisted polycistronic co-expression system：pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.

Usage and Biology

We constructed SAHS 33020 into pRAP to endow E. coli with desiccation-resistant and anti-UV capability. And this composite part can cooperate with ribozyme+T7_RBS+SAHS 33020+stem-loop to increase the survival rate of E.coli under desiccation.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 247
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 515
Illegal BsaI.rc site found at 596
Illegal SapI site found at 409

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4765113 short</partinfo>
-ribozyme+T7_RBS+H. ex mtSSB+stem-loop
+===Introduction===
+This composite part is composed of ''Hypsibius exemplaris'' mitochondrial single-stranded DNA binding protein (H. ex mtSSB) and ribozyme-assisted polycistronic co-expression system：pRAP. H. ex mtSSB can coat ssDNA to limit damage during desiccation and UV radiation.
+===Usage and Biology===
+We constructed SAHS 33020 into pRAP to endow ''E. coli'' with desiccation-resistant and anti-UV capability. And this composite part can cooperate with [https://parts.igem.org/Part:BBa_K4765112 ribozyme+T7_RBS+SAHS 33020+stem-loop] to increase the survival rate of ''E.coli'' under desiccation.
+===Characterization===
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 23: @@
 <partinfo>BBa_K4765113 parameters</partinfo>
 <!-- -->
+==Reference==