Difference between revisions of "Part:BBa K4579008"
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We chose to design the CvaC15 as its own part rather than include it with the microcin in order to ensure that (1) any microcin, regardless of what bacterium it originated from, would be secreted by the E. coli T1SS encoded by the Davies Lab secretion system plasmid, and (2) the secretion system does not need to be changed for each microcin. All known microcins have a signal peptide sequence, but this can differ from microcin to microcin depending on the specific variants of the secretion system genes found within the genome of the bacterium from which the microcin originates. As such, by designing the signal peptide as its own assembly part, any microcin or other small peptide can be swapped in and out of the system with the confidence that it will always be fused to the T1SS-compatible signal peptide when translated. The CvaC15 signal peptide was nested in a high copy entry vector with a ColE1 origin of replication with chloramphenicol resistance. | We chose to design the CvaC15 as its own part rather than include it with the microcin in order to ensure that (1) any microcin, regardless of what bacterium it originated from, would be secreted by the E. coli T1SS encoded by the Davies Lab secretion system plasmid, and (2) the secretion system does not need to be changed for each microcin. All known microcins have a signal peptide sequence, but this can differ from microcin to microcin depending on the specific variants of the secretion system genes found within the genome of the bacterium from which the microcin originates. As such, by designing the signal peptide as its own assembly part, any microcin or other small peptide can be swapped in and out of the system with the confidence that it will always be fused to the T1SS-compatible signal peptide when translated. The CvaC15 signal peptide was nested in a high copy entry vector with a ColE1 origin of replication with chloramphenicol resistance. | ||
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Revision as of 20:56, 30 September 2023
CvaC15 - Signal peptide
We chose to design the CvaC15 as its own part rather than include it with the microcin in order to ensure that (1) any microcin, regardless of what bacterium it originated from, would be secreted by the E. coli T1SS encoded by the Davies Lab secretion system plasmid, and (2) the secretion system does not need to be changed for each microcin. All known microcins have a signal peptide sequence, but this can differ from microcin to microcin depending on the specific variants of the secretion system genes found within the genome of the bacterium from which the microcin originates. As such, by designing the signal peptide as its own assembly part, any microcin or other small peptide can be swapped in and out of the system with the confidence that it will always be fused to the T1SS-compatible signal peptide when translated. The CvaC15 signal peptide was nested in a high copy entry vector with a ColE1 origin of replication with chloramphenicol resistance.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]