Difference between revisions of "Part:BBa K4765107"
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| + | __NOTOC__ | ||
| + | <partinfo>BBa_K4765107 short</partinfo> | ||
| + | __TOC__ | ||
| + | ===introduction=== | ||
| + | INPNC-Ag3 fusion is composed of a surface display system (INPNC+linker) and the coding sequence of a nanobody. INPNC exhibits compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers<ref>van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003</ref>.Ag3 is a corresponding antigen of [https://parts.igem.org/Part:BBa_K4765007 Nb3]<ref>Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041 | ||
| + | </ref>. The interaction between Ag-Nb can mediate specific adhesion of Escherichia coli. A flexible protein domain linker of 10 aa was introduced between INPNC and Ag3 to ensure independent functionality of Ag3 and INPNC with minimal mutual disruption. | ||
| + | We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP. | ||
| + | ===Usage and Biology=== | ||
| + | The surface-displayed antigen can specifically interact with the nanobody produced by [https://parts.igem.org/Part:BBa_K4765108 ribozyme+strong RBS+INPNC-Nb3 fusion+stem-loop]. | ||
| + | ===Characterization=== | ||
Revision as of 12:42, 30 September 2023
Twister P1 + T7_RBS + INPNC-Ag3 fusion + stem-loop
introduction
INPNC-Ag3 fusion is composed of a surface display system (INPNC+linker) and the coding sequence of a nanobody. INPNC exhibits compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers[1].Ag3 is a corresponding antigen of Nb3[2]. The interaction between Ag-Nb can mediate specific adhesion of Escherichia coli. A flexible protein domain linker of 10 aa was introduced between INPNC and Ag3 to ensure independent functionality of Ag3 and INPNC with minimal mutual disruption. We’ve constructed this fusion protein into our ribozyme-assisted polycistronic co-expression system：pRAP.
Usage and Biology
The surface-displayed antigen can specifically interact with the nanobody produced by ribozyme+strong RBS+INPNC-Nb3 fusion+stem-loop.
Characterization
- ↑ van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003
- ↑ Glass, D. S., & Riedel-Kruse, I. H. (2018). A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns. Cell, 174(3), 649-658.e16. https://doi.org/10.1016/j.cell.2018.06.041