Difference between revisions of "Part:BBa K4650003"
Michael5186 (Talk | contribs) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4650003 short</partinfo> | <partinfo>BBa_K4650003 short</partinfo> | ||
| + | <h4> | ||
| − | MaSp2 encodes for spider silk protein, which is a highly repetitive amino acid sequence and has the properties of being strong, flexible, and elastic. This amino acid sequence is relatively short compared to other amino acid sequences for spider silk proteins listed in the paper by Tian et al, 2003. The properties of MaSp2 in our project are to make the scoby membrane more flexible and softer. Our team converted this amino acid sequence into DNA sequences and consulted with the Mission Biotech company where we ordered the MaSp1(BBa_K4650002) and MaSp2 from. The company suggested our team to use several different 3-nucleotide codons encoded for one amino acid in these repeated amino acid sequences, so we would not get the same repeated DNA sequence. After consultation by the company, the team has made those MaSp1, and MaSp2 synthetic DNA along with the functional primer sets for MaSp1 and MaSp2 PCR technique. Our team also added the start codon, ATG, at the 5'end of the MaSp2 DNA sequence so the RNA transcription would start from ATG on MaSp2 DNA. | + | MaSp2 encodes for spider silk protein, which is a highly repetitive amino acid sequence and has the properties of being strong, flexible, and elastic. This amino acid sequence is relatively short compared to other amino acid sequences for spider silk proteins listed in the paper by Tian et al, 2003. The properties of MaSp2 in our project are to make the scoby membrane more flexible and softer. Our team converted this amino acid sequence into DNA sequences and consulted with the Mission Biotech company where we ordered the MaSp1(BBa_K4650002) and MaSp2 from. The company suggested our team to use several different 3-nucleotide codons encoded for one amino acid in these repeated amino acid sequences, so we would not get the same repeated DNA sequence. After consultation by the company, the team has made those MaSp1, and MaSp2 synthetic DNA along with the functional primer sets for MaSp1 and MaSp2 PCR technique. Our team also added the start codon, ATG, at the 5'end of the MaSp2 DNA sequence so the RNA transcription would start from ATG on MaSp2 DNA. <br><br> |
| − | Tian M. Expression and Purification of A Synthetic Spider Silk Protein In Arabidopsis thaliana. Master thesis. University of Wyoming; 2003. | + | Citation: Tian M. Expression and Purification of A Synthetic Spider Silk Protein In Arabidopsis thaliana. Master thesis. University of Wyoming; 2003.<br><br> |
| + | <span class='h3bb'>Sequence and Features</span> | ||
| + | </h4> | ||
| + | |||
| + | https://static.igem.wiki/teams/4650/wiki/parts-image/bba-k4650003-masp2-pcr.jpg | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 12: | Line 18: | ||
<!-- --> | <!-- --> | ||
| − | + | ||
<partinfo>BBa_K4650003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4650003 SequenceAndFeatures</partinfo> | ||
Latest revision as of 08:36, 30 September 2023
spider silk protein MaSp2
MaSp2 encodes for spider silk protein, which is a highly repetitive amino acid sequence and has the properties of being strong, flexible, and elastic. This amino acid sequence is relatively short compared to other amino acid sequences for spider silk proteins listed in the paper by Tian et al, 2003. The properties of MaSp2 in our project are to make the scoby membrane more flexible and softer. Our team converted this amino acid sequence into DNA sequences and consulted with the Mission Biotech company where we ordered the MaSp1(BBa_K4650002) and MaSp2 from. The company suggested our team to use several different 3-nucleotide codons encoded for one amino acid in these repeated amino acid sequences, so we would not get the same repeated DNA sequence. After consultation by the company, the team has made those MaSp1, and MaSp2 synthetic DNA along with the functional primer sets for MaSp1 and MaSp2 PCR technique. Our team also added the start codon, ATG, at the 5'end of the MaSp2 DNA sequence so the RNA transcription would start from ATG on MaSp2 DNA.
Citation: Tian M. Expression and Purification of A Synthetic Spider Silk Protein In Arabidopsis thaliana. Master thesis. University of Wyoming; 2003.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]