Difference between revisions of "Part:BBa K4789000:Design"

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===Design Notes===
 
===Design Notes===
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Refer to Yang, et.al 2019.
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miR-22-sponge was designed according to the binding site between miR-22 and lncRNA MALAT1.
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===Source===
 
===Source===
  
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The plasmid of pCDNA3-MCS-nPepper was obtained from Naying Biotechnology Company.
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The miR-22-sponge were synthesized by Nanjing Genscript Biotechnology corporation.
  
 
===References===
 
===References===
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Chen Xianjun,Zhang Dasheng,Su Ni et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.[J] .Nat Biotechnol, 2019, 37: 1287-1293.
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1.Han Xiting,Wang Qian,Wang Yan et al. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1/microRNA-202-3p/periostin axis modulates invasion and epithelial-mesenchymal transition in human cervical cancer.[J] .J Cell Physiol, 2019, 234: 14170-14180.
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2.Shen Fujin,Zheng Hongyun,Zhou Limei et al. Overexpression of MALAT1 contributes to cervical cancer progression by acting as a sponge of miR-429.[J] .J Cell Physiol, 2019, 234: 11219-11226.
 +
 
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3.Aftab Mehreen,Poojary Satish S,Seshan Vaishnavi et al. Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer.[J] .Sci Rep, 2021, 11: 10323.
 +
 
 +
4.Chen Mingming,Ma Zhao,Wu Xiaotian et al. A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.[J] .Sci Rep, 2017, 7: 1550.
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5.Kwon Ah-Young,Jeong Ju-Yeon,Park Hyun et al. miR-22-3p and miR-30e-5p Are Associated with Prognosis in Cervical Squamous Cell Carcinoma.[J] .Int J Mol Sci, 2022, 23: undefined.

Latest revision as of 06:38, 30 September 2023


miR-22-sponge


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

miR-22-sponge was designed according to the binding site between miR-22 and lncRNA MALAT1.

Source

The miR-22-sponge were synthesized by Nanjing Genscript Biotechnology corporation.

References

1.Han Xiting,Wang Qian,Wang Yan et al. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1/microRNA-202-3p/periostin axis modulates invasion and epithelial-mesenchymal transition in human cervical cancer.[J] .J Cell Physiol, 2019, 234: 14170-14180.

2.Shen Fujin,Zheng Hongyun,Zhou Limei et al. Overexpression of MALAT1 contributes to cervical cancer progression by acting as a sponge of miR-429.[J] .J Cell Physiol, 2019, 234: 11219-11226.

3.Aftab Mehreen,Poojary Satish S,Seshan Vaishnavi et al. Urine miRNA signature as a potential non-invasive diagnostic and prognostic biomarker in cervical cancer.[J] .Sci Rep, 2021, 11: 10323.

4.Chen Mingming,Ma Zhao,Wu Xiaotian et al. A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.[J] .Sci Rep, 2017, 7: 1550.

5.Kwon Ah-Young,Jeong Ju-Yeon,Park Hyun et al. miR-22-3p and miR-30e-5p Are Associated with Prognosis in Cervical Squamous Cell Carcinoma.[J] .Int J Mol Sci, 2022, 23: undefined.