Difference between revisions of "Part:BBa K4937020:Design"

Latest revision as of 08:44, 28 September 2023

OAZ1_upstream-TEF1p-SPE1-PRM9t-TDH3p-AtACL5-DIT1t-OAZ1_downstream

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2336
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2072
Illegal BglII site found at 5129
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 632

Design Notes

.

Source

This part is from fusion PCR of BBa_K4937016, BBa_K4937000, BBa_K4937012, BBa_K4937005, BBa_K4937009, BBa_K4937001, BBa_K4937012, BBa_K4937008, BBa_K4937010, and BBa_K4937017.

References

Qin, J., Krivoruchko, A., Ji, B. et al. Engineering yeast metabolism for the discovery and production of polyamines and polyamine analogues. Nat Catal 4, 498–509 (2021). https://doi.org/10.1038/s41929-021-00631-z

Hamilton, R., Watanabe, C. K., & de Boer, H. A. (1987). Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic acids research, 15(8), 3581–3593. https://doi.org/10.1093/nar/15.8.3581

@@ Line 13: / Line 13: @@
 ===Source===
-.
+This part is from fusion PCR of BBa_K4937016, BBa_K4937000, BBa_K4937012, BBa_K4937005, BBa_K4937009, BBa_K4937001, BBa_K4937012, BBa_K4937008,  BBa_K4937010, and BBa_K4937017.
 ===References===
+<p>Qin, J., Krivoruchko, A., Ji, B. et al. Engineering yeast metabolism for the discovery and production of polyamines and polyamine analogues. Nat Catal 4, 498–509 (2021). https://doi.org/10.1038/s41929-021-00631-z</p>
+<p>Hamilton, R., Watanabe, C. K., & de Boer, H. A. (1987). Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic acids research, 15(8), 3581–3593. https://doi.org/10.1093/nar/15.8.3581</p>