Difference between revisions of "Part:BBa K4937019:Design"

 
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===References===
 
===References===
 +
<p>Qin, J., Krivoruchko, A., Ji, B. et al. Engineering yeast metabolism for the discovery and production of polyamines and polyamine analogues. Nat Catal 4, 498–509 (2021). https://doi.org/10.1038/s41929-021-00631-z</p>
 +
Hamilton, R., Watanabe, C. K., & de Boer, H. A. (1987). Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic acids research, 15(8), 3581–3593. https://doi.org/10.1093/nar/15.8.3581

Revision as of 06:12, 28 September 2023


OAZ1_upstream-TEF1p-SPE1-PRM9t-OAZ1_downstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2336
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2072
    Illegal BglII site found at 2993
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 632


Design Notes

.


Source

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References

Qin, J., Krivoruchko, A., Ji, B. et al. Engineering yeast metabolism for the discovery and production of polyamines and polyamine analogues. Nat Catal 4, 498–509 (2021). https://doi.org/10.1038/s41929-021-00631-z

Hamilton, R., Watanabe, C. K., & de Boer, H. A. (1987). Compilation and comparison of the sequence context around the AUG startcodons in Saccharomyces cerevisiae mRNAs. Nucleic acids research, 15(8), 3581–3593. https://doi.org/10.1093/nar/15.8.3581