Difference between revisions of "Part:BBa K4650001"
 
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<partinfo>BBa_K4650001 short</partinfo>
 
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The cellulose-binding domain (CBD) proteins are proven to lead other proteins to cellulose in both vivo and vitro. In several organisms, such as yeast, plants, and bacteria, They are used to help protein complexes to bind to cellulose for other catalytic activities. For laboratory applications, it is used for in vitro protein purification as only their target gene fused with CBD would bind to the immobilized cellulose via washing through the column. The CBM1 gene in fission yeast,  S. pombe, is one of the genes encoded for the CBD family. The purpose of cloning this CBM1(=CBD) is to carry our spider silk proteins, MaSp1, and MaSp2 to bind to scoby membranes.  
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<h4>
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The cellulose-binding domain (CBD) proteins are proven to lead other proteins to cellulose in both vivo and vitro. In several organisms, such as yeast, plants, and bacteria, They are used to help protein complexes to bind to cellulose for other catalytic activities. For laboratory applications, it is used for in vitro protein purification as only their target gene fused with CBD would bind to the immobilized cellulose via washing through the column. The CBM1 gene in fission yeast,  S. pombe, is one of the genes encoded for the CBD family. The purpose of cloning this CBM1(=CBD) is to carry our spider silk proteins, MaSp1, and MaSp2 to bind to scoby membranes. <br><br>
  
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Doi RH, Park JS, Shin HS. Fusion proteins containing cellulose-binding domains. Methods in Enzymology. 2000;326: 418-429. Available from: doi:10.1016/S0076-6879(00)26067-9.
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Doi RH, Park JS, Shin HS. Fusion proteins containing cellulose-binding domains. Methods in Enzymology. 2000;326: 418-429. Available from: doi:10.1016/S0076-6879(00)26067-9. <br><br>
  
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<span class='h3bb'>Sequence and Features</span>
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</h4>
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https://static.igem.wiki/teams/4650/wiki/parts-image/bba-k4650001-cbd-pcr.jpg
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4650001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4650001 SequenceAndFeatures</partinfo>
  

Latest revision as of 14:34, 27 September 2023


cellulose binding domain (CBD)

The cellulose-binding domain (CBD) proteins are proven to lead other proteins to cellulose in both vivo and vitro. In several organisms, such as yeast, plants, and bacteria, They are used to help protein complexes to bind to cellulose for other catalytic activities. For laboratory applications, it is used for in vitro protein purification as only their target gene fused with CBD would bind to the immobilized cellulose via washing through the column. The CBM1 gene in fission yeast, S. pombe, is one of the genes encoded for the CBD family. The purpose of cloning this CBM1(=CBD) is to carry our spider silk proteins, MaSp1, and MaSp2 to bind to scoby membranes.

Doi RH, Park JS, Shin HS. Fusion proteins containing cellulose-binding domains. Methods in Enzymology. 2000;326: 418-429. Available from: doi:10.1016/S0076-6879(00)26067-9.

Sequence and Features

bba-k4650001-cbd-pcr.jpg



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 499
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 268
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 499
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 499
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 51