Difference between revisions of "Part:BBa K4727111"
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<partinfo>BBa_K4727111 short</partinfo> | <partinfo>BBa_K4727111 short</partinfo> | ||
| − | This guide DNA has been designed to work with dCas9 to target AMR related genes in | + | This guide DNA has been designed to work with dCas9 to target AMR related genes in <i> Klebsiella pneumoniae </i>. The guide binds 181bp downstream the TSS. |
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| + | The penicillin resistance gene blaSHV-1 was first discovered in <i>K. pneumoniae</i> in the 1960s. It is a class A beta-lactamase and also has β-lactamase activity against cephalosporins. It determines resistance against ampicillin. | ||
| + | Over the years, many genes for broad-spectrum β-lactamases have developed, and have been identified, in various clinical strains of <i>K. pneumoniae</i>, increasing the emergence of infections caused by this bacterium. | ||
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Latest revision as of 12:24, 27 September 2023
sgDNA BlaSHV-1 181 in K. pneumoniae
This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae . The guide binds 181bp downstream the TSS.
The penicillin resistance gene blaSHV-1 was first discovered in K. pneumoniae in the 1960s. It is a class A beta-lactamase and also has β-lactamase activity against cephalosporins. It determines resistance against ampicillin. Over the years, many genes for broad-spectrum β-lactamases have developed, and have been identified, in various clinical strains of K. pneumoniae, increasing the emergence of infections caused by this bacterium.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]