Difference between revisions of "Part:BBa K4727108"
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<partinfo>BBa_K4727108 short</partinfo> | <partinfo>BBa_K4727108 short</partinfo> | ||
| − | This guide DNA has been designed to work with dCas9 to target AMR related genes in | + | This guide DNA has been designed to work with dCas9 to target AMR related genes in <i> Acinetobacter baumannii </i>. The guide has been designed to bins 148bp downstream the TSS |
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| + | blaADC, also called blaAmpC, is a class C β-lactamase, i.e. a cephalosporinase encoded by the chromosome in A. baumannii. It involves resistance to Ampicillin, especially when it is overexpressed due to the insertion of short transposon sequences, which are stronger promoters than the intrinsic one. | ||
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Latest revision as of 12:12, 27 September 2023
sgDNA AmpC 148
This guide DNA has been designed to work with dCas9 to target AMR related genes in Acinetobacter baumannii . The guide has been designed to bins 148bp downstream the TSS
blaADC, also called blaAmpC, is a class C β-lactamase, i.e. a cephalosporinase encoded by the chromosome in A. baumannii. It involves resistance to Ampicillin, especially when it is overexpressed due to the insertion of short transposon sequences, which are stronger promoters than the intrinsic one.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]