Difference between revisions of "Part:BBa K4650001"

Revision as of 01:05, 27 September 2023

cellulose binding domain (CBD)

The cellulose-binding domain (CBD) proteins are proven to lead other proteins to cellulose in both vivo and vitro. In several organisms, such as yeast, plants, and bacteria, They are used to help protein complexes to bind to cellulose for other catalytic activities. For laboratory applications, it is used for in vitro protein purification as only their target gene fused with CBD would bind to the immobilized cellulose via washing through the column. The CBM1 gene in fission yeast, S. pombe, is one of the genes encoded for the CBD family. The purpose of cloning this CBM1(=CBD) is to carry our spider silk proteins, MaSp1, and MaSp2 to bind to scoby membranes.

Doi RH, Park JS, Shin HS. Fusion proteins containing cellulose-binding domains. Methods in Enzymology. 2000;326: 418-429. Available from: doi:10.1016/S0076-6879(00)26067-9.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 499
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 499
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 268
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 499
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 499
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 51

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4650001 short</partinfo>
-Cellulose binding domain proteins (CBDs) are contained in several organisms, such as fungi, bacteria, and plants, and the major function of CBDs in those organisms is to help the enzymes or the complex bind to the cellulose for further catalytic activities beneficial for the in vivo cellular processes
+The cellulose-binding domain (CBD) proteins are proven to lead other proteins to cellulose in both vivo and vitro. In several organisms, such as yeast, plants, and bacteria, They are used to help protein complexes to bind to cellulose for other catalytic activities. For laboratory applications, it is used for in vitro protein purification as only their target gene fused with CBD would bind to the immobilized cellulose via washing through the column. The CBM1 gene in fission yeast,  S. pombe, is one of the genes encoded for the CBD family.
+The purpose of cloning this CBM1(=CBD) is to carry our spider silk proteins, MaSp1, and MaSp2 to bind to scoby membranes.
+ Doi RH, Park JS, Shin HS. Fusion proteins containing cellulose-binding domains. Methods in Enzymology. 2000;326: 418-429. Available from: doi:10.1016/S0076-6879(00)26067-9.
 <!-- Add more about the biology of this part here