Difference between revisions of "Part:BBa K185049"

Revision as of 09:42, 17 October 2009

Lon protease

The ATP-dependent Lon protease is a member of the AAA+ family of proteins (ATPases associated with various cellular activities), which is present in archaea, prokaryotes and eukaryotic mitochondria, peroxisomes and plastids. Lon has multiple cellular functions such as degrading abnormal polypeptides, as well as certain regulatory proteins and metabolic enzymes, acting as a chaperone and binding to nucleic acids. In our project, since the Lon protease can hydrolysis RelB protein efficiently, we use it to balance the concentration of relE and relB inside E.coli. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 619
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 619
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1332
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 619
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 619
Illegal AgeI site found at 1795
Illegal AgeI site found at 1879
Illegal AgeI site found at 2077
Illegal AgeI site found at 2110
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K185049 short</partinfo>
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+The ATP-dependent Lon protease is a member of the AAA+ family of proteins (ATPases associated with various cellular activities), which is present in archaea, prokaryotes and eukaryotic mitochondria, peroxisomes and plastids. Lon has multiple cellular functions such as degrading abnormal polypeptides, as well as certain regulatory proteins and metabolic enzymes, acting as a chaperone and binding to nucleic acids.
+In our project, since the Lon protease can hydrolysis RelB protein efficiently, we use it to balance the concentration of relE and relB inside E.coli.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===