Difference between revisions of "Part:BBa K4824000:Experience"
Line 5: Line 5:
  
 
===Applications of BBa_K4824000===
 
===Applications of BBa_K4824000===
 +
=Introduction=
 +
Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.
  
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K4824000 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4824000 StartReviews</partinfo>
 
<!-- Template for a user review
 
<!-- Template for a user review
−
=Introduction=
+
 
−
Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.
+
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'
 
|-
 
|-

Revision as of 06:35, 25 September 2023


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4824000

Introduction

Bioparts are the basic building blocks of the iGEM project. Here, we constructed a catalytic lignan glycoside (BBa_K4824000) biopart (Figure 1). Based on the reported transcriptomic data of I. indigotica, the sequences of genes with glycosyltransferase functions were screened and a phylogenetic tree analysis was conducted with the functionally characterized lignan UGTs, and UGT72B2 was selected as the candidate, which the main component of the biopart. We then constructed expression vectors using homologous recombination and ultimately verified protein function.

User Reviews

UNIQ4faae3a7a0f2d07b-partinfo-00000000-QINU UNIQ4faae3a7a0f2d07b-partinfo-00000001-QINU